Qiagen Webinar on real-time qPCR

1. Mon, Mar 7, 2016 8:00 PM – 9:00 PM EET
2. Mon, Mar 14, 2016 7:00 PM – 8:00 PM EET
3. Mon, Mar 21, 2016 7:00 PM – 8:00 PM EET

Part 1: Critical factors for real-time qPCR: RNA quality controls and RT steps
Mar 7, 1-2 pm EST

In this webinar we will cover the following topics which are critical steps for efficient and precise gene expression studies using real-time PCR technology:
– Effect of RNA integrity on real-time PCR results – tips to achieve a true RNA profiling suitable for real-time PCR studies
– Improved methods for cDNA synthesis, optimized for real-time PCR
– Factors influencing specificity of real-time PCR analysis

Part 2: The importance of controls and novel solutions for successful real-time qPCR
Mar 14

The increasing demand for streamlined, monitored, and ultrafast qPCR procedures requires high-performance, real-time quantitative RT and PCR chemistries. This webinar presents innovative solutions for one-step and two-step RT-PCR that significantly enhance performance and reliability in qRT-PCR. Internal Control RNA, removal of genomic DNA, room temperature set up capability for RT-PCR, and a built-in visual pipetting control verify accurate procedures, ensuring reliable gene expression profiling.
This webinar explains the principles of the technologies and shows data demonstrating performance in qRT-PCR. Find out how you can verify accurate performance in qRT-PCR and improve your results.

Part 3: Interpret Your Gene Expression Analysis Results with Advanced Bioinformatics Tools
Mar 21
You’ve got raw Ct data and fold changes from your gene expression analysis experiment. How do you make sense of the results to build a model that explains the biology and helps you design the next best experiment?

In this webinar, we will introduce a gene expression workflow and an easy-to-use yet advanced Ingenuity Pathway Analysis (IPA) bioinformatics tool. With a case study, we will explore the potential that RT-PCR array and bioinformatics combined to help understanding the regulatory roles of some genes in diseases and get better insight into the biological function and find potential biomarkers.